THE reaction of iminazoles with diazobenzene sulphonic acid, which was introduced into protein chemistry by Later Koessler and Hanke [1919] elaborated their colori- metric method for the determination of histidine in protein hydrolysates. The diazo-compound formed was also determined by volumetric titration by The compound was reduced by titanous chloride, and the excess of titanous salt was oxidised by ferric alum, thiocyanate being used as indicator.
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Erik Jorpes (1932) studied this question.
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