What question did this study set out to answer?

To evaluate the genomic diversity of Staphylococcus aureus coagulase type VII strains associated with food poisoning outbreaks and determine the epidemiological utility of pulsed-field gel electrophoresis.

October 1, 2000Journal of Clinical MicrobiologyOpen Access

Characterization ofStaphylococcus aureusCoagulase Type VII Isolates from Staphylococcal Food Poisoning Outbreaks (1980–1995) in Tokyo, Japan, by Pulsed-Field Gel Electrophoresis

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To evaluate the genomic diversity of Staphylococcus aureus coagulase type VII strains associated with food poisoning outbreaks and determine the epidemiological utility of pulsed-field gel electrophoresis.
Analyzed 129 Staphylococcus aureus coagulase type VII isolates from 129 food poisoning outbreak cases across 35 districts over a 16-year period (1980–1995) in Tokyo, Japan.
Digested bacterial chromosomal DNA using the restriction enzyme SmaI and resolved fragments via pulsed-field gel electrophoresis (PFGE) to establish genomic types and subtypes.
Classified the 129 isolates into three major PFGE genomic types: type A (n=115), type B (n=10), and type C (n=4).
Further differentiated type A and type C isolates into 33 subtypes (A1 to A33) and 4 subtypes (C1 to C4), respectively.
Identified identical PFGE subtypes recurring in the same geographical districts across intervals of 1 to 5 years.

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Implication

Epidemiological study reveals genetic diversity among Staphylococcus aureus outbreak strains, highlighting pulsed-field gel electrophoresis for foodborne illness tracking.

Cite This Study

Shimizu et al. (2000) studied this question.

synapsesocial.com/papers/6a8a8b95af6b08ee8d723f6f https://doi.org/10.1128/jcm.38.10.3746-3749.2000

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Implication

Epidemiological study reveals genetic diversity among Staphylococcus aureus outbreak strains, highlighting pulsed-field gel electrophoresis for foodborne illness tracking.

Characterization ofStaphylococcus aureusCoagulase Type VII Isolates from Staphylococcal Food Poisoning Outbreaks (1980–1995) in Tokyo, Japan, by Pulsed-Field Gel Electrophoresis

Key Points

Discussion

Implication

Cite This Study

Characterization ofStaphylococcus aureusCoagulase Type VII Isolates from Staphylococcal Food Poisoning Outbreaks (1980–1995) in Tokyo, Japan, by Pulsed-Field Gel Electrophoresis

Key Points

Discussion

Implication

Cite This Study

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