AbstractAimTo evaluate the effect of alkaline phosphatase (ALP) immobilization onto Bio‐Gide® in vitro, and to study the in vivo performance of ALP‐enriched Bio‐Gide® and/or Bio‐Oss® with the purpose to enhance periodontal regeneration.Materials and MethodsAlkaline phosphatase ALP was immobilized onto Bio‐Gide® and Bio‐Oss®. Forty‐eight rats received periodontal defects, which were treated according to one of the following strategies: Bio‐Gide®, Bio‐Gide®‐ALP, Bio‐Gide®‐ALP/Bio‐Oss®, Bio‐Gide®/Bio‐Oss®‐ALP, Bio‐Gide®‐ALP/Bio‐Oss®‐ALP, or empty. Micro‐CT and histological analysis were performed.ResultsA 30 min ALP‐deposition time was determined as optimal from mineralization capacity assessment and consequently used as Bio‐Gide®‐ALP membranes in the animal experiment. In vivo results showed that after 2 weeks, the defect and implanted materials were still visible, an inflammatory response was present, and membrane degradation was ongoing. Bone formation, although limited, was observed in the majority of Bio‐Gide®‐ALP specimens and all of the Bio‐Gide®/Bio‐Oss®‐ALP specimens, and was significantly higher compared with Bio‐Gide® and empty controls. After 6 weeks, the defects and particles were still visible, whereas membranes were completely degraded. The inflammatory response was decreased and bone formation appeared superior for Bio‐Gide®‐ALP treated defects.ConclusionImmobilization of ALP onto guided tissue regeneration (GTR)/ guided bone regeneration (GBR)‐materials (Bio‐Gide® and Bio‐Oss®) can enhance the performance of these materials in GTR/GBR procedures.
No takes yet. Share an insight, caveat, or question.
Oortgiesen et al. (2012) studied this question.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: