What question did this study set out to answer?

To identify signaling proteins that interact with the inhibitory receptor CD5 following thymocyte activation.

May 1, 1998European Journal of Immunology

Thymocyte activation induces the association of the proto-oncoprotein c-cbl and ras GTPase- activating protein with CD5

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Authors

KDKevin M. DennehyUniversity of Augsburg RBRichard BroszeitStellenbosch University WFWilliam Frank FerrisStellenbosch University

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Implication

Experimental study reveals recruitment of c-cbl and ras GAP to CD5 in activated thymocytes, indicating a mechanism for down-regulating antigen receptor signaling.

Key Points

To identify signaling proteins that interact with the inhibitory receptor CD5 following thymocyte activation.
Stimulated thymocytes using CD5 cross-linking or pervanadate treatment to induce tyrosine phosphorylation.
Identified CD5-interacting proteins via immunoprecipitation, reprecipitation assays, and phosphopeptide binding analysis.
Pervanadate stimulation and CD5 cross-linking induced association of a 120-kDa phosphoprotein with CD5, which reprecipitation confirmed to be predominantly the proto-oncoprotein c-cbl.
Ras GTPase-activating protein (ras GAP) associated with CD5 in stimulated thymocytes through an SH2-mediated interaction with the phosphorylated Y429SQP motif of CD5.

Cite This Study

Dennehy et al. (1998) studied this question.

synapsesocial.com/papers/6a8aa0fc451a22fc7efb9a04 https://doi.org/10.1002/(sici)1521-4141(199805)28:05<1617::aid-immu1617>3.0.co;2-7

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Implication

Experimental study reveals recruitment of c-cbl and ras GAP to CD5 in activated thymocytes, indicating a mechanism for down-regulating antigen receptor signaling.

Thymocyte activation induces the association of the proto-oncoprotein c-cbl and ras GTPase- activating protein with CD5

Discussion

Member takes

Implication

Key Points

Cite This Study

Thymocyte activation induces the association of the proto-oncoprotein c-cbl and ras GTPase- activating protein with CD5

Discussion

Member takes

Implication

Key Points

Cite This Study

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