What does this research mean for the field?

Following global ischemia/reperfusion injury, oxidative stress peaks in the early phase, while the expression of transcription factors C/EBPβ and SREBP-1 increases during the early-to-mid and mid-phases of neurodegeneration, respectively. Novelty: ClaimNovelty.INCREMENTAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

To investigate the temporal expression profiles of transcription factors SREBP-1 and C/EBPβ alongside oxidative stress markers in rat brain cortex and cerebellum following global ischemia/reperfusion injury.

August 23, 2026Turkish Journal of BiochemistryOpen Access

Investigation of SREBP-1 and C/EBPβ expression during global ischemia/reperfusion-induced oxidative stress in rat brain cortex and cerebellum

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Authors

MDMelih Dağdeviren MIMesude Iscan PKPelin Kelicen‐Uğur

Discussion

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Overview

Animal experiment reveals timed increases in SREBP-1, C/EBPβ, and oxidative stress markers in rat brains following ischemia/reperfusion, indicating phase-specific roles in neurodegeneration.

Key Points

To investigate the temporal expression profiles of transcription factors SREBP-1 and C/EBPβ alongside oxidative stress markers in rat brain cortex and cerebellum following global ischemia/reperfusion injury.
Adult male rats underwent 10 minutes of carotid artery occlusion and hypotension (or sham operation), followed by reperfusion periods of 24 hours, 1 week, 2 weeks, or 4 weeks.
Quantified SREBP-1 and C/EBPβ protein levels in cytosolic and nuclear fractions using Western blotting.
Measured oxidative stress parameters, including TBARS and total thiol levels alongside SOD and GST enzymatic activities.
C/EBPβ expression increased significantly in cortical cytosolic (1.19- to 1.58-fold) and nuclear (1.73- to 1.81-fold) extracts at 24 hours and 1 week, and in cerebellar extracts at 1 week (1.63-fold cytosolic, 1.35-fold nuclear).
SREBP-1 expression peaked at 1 week post-reperfusion across cortical (2.07-fold cytosolic, 1.41-fold nuclear) and cerebellar (2.15-fold cytosolic, 1.79-fold nuclear) fractions.
TBARS levels and SOD activities rose significantly by 43.16% and 47.30%, respectively, at 24 hours post-reperfusion.

Cite This Study

Dağdeviren et al. (2026) studied this question.

synapsesocial.com/papers/6a8aadf37677a341144467f2 https://doi.org/10.1515/tjb-2025-0362

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August 23, 2026Turkish Journal of BiochemistryOpen Access

Investigation of SREBP-1 and C/EBPβ expression during global ischemia/reperfusion-induced oxidative stress in rat brain cortex and cerebellum

View Full Paper

Ask AI

Bookmark

Authors

MDMelih Dağdeviren MIMesude Iscan PKPelin Kelicen‐Uğur

Discussion

Member takes

Overview

Animal experiment reveals timed increases in SREBP-1, C/EBPβ, and oxidative stress markers in rat brains following ischemia/reperfusion, indicating phase-specific roles in neurodegeneration.

Key Points

To investigate the temporal expression profiles of transcription factors SREBP-1 and C/EBPβ alongside oxidative stress markers in rat brain cortex and cerebellum following global ischemia/reperfusion injury.
Adult male rats underwent 10 minutes of carotid artery occlusion and hypotension (or sham operation), followed by reperfusion periods of 24 hours, 1 week, 2 weeks, or 4 weeks.
Quantified SREBP-1 and C/EBPβ protein levels in cytosolic and nuclear fractions using Western blotting.
Measured oxidative stress parameters, including TBARS and total thiol levels alongside SOD and GST enzymatic activities.
C/EBPβ expression increased significantly in cortical cytosolic (1.19- to 1.58-fold) and nuclear (1.73- to 1.81-fold) extracts at 24 hours and 1 week, and in cerebellar extracts at 1 week (1.63-fold cytosolic, 1.35-fold nuclear).
SREBP-1 expression peaked at 1 week post-reperfusion across cortical (2.07-fold cytosolic, 1.41-fold nuclear) and cerebellar (2.15-fold cytosolic, 1.79-fold nuclear) fractions.
TBARS levels and SOD activities rose significantly by 43.16% and 47.30%, respectively, at 24 hours post-reperfusion.

Cite This Study

Dağdeviren et al. (2026) studied this question.

synapsesocial.com/papers/6a8aadf37677a341144467f2 https://doi.org/10.1515/tjb-2025-0362

View Full Paper

Ask AI

Bookmark