Microfluidic chip‐based liquid chromatography coupled to mass spectrometry for determination of small molecules in bioanalytical applications: A n update

Many microfluidic chip‐based LC‐MS systems have been utilized for high‐throughput analysis in various fields of bioanalytical applications such as proteomic, glycomic, pharmaceutical, and clinical research. This review is an update of a previous review article (Electrophoresis 2012, 33, 635–643) to mainly cover the most recent advancements in chip‐based LC‐MS for determining small molecules in bioanalysis. First, the different types of microfluidic chip devices for chip‐based LC‐MS analysis will be discussed. Following the discussion of the recent developments in the chip‐based instrumentation, the applications of chip‐based LC‐MS for determining small molecules, such as glycans, pharmaceutical drugs, drugs of abuse, drug metabolites, and biomarkers in various biological sample matrixes will also be included in this review.