Human brain‐specific alpha2‐glycoprotein was purified by means of Sepharose immunoadsorbents. A further brain‐specific protein was found by this method. This component appears to be present in brain in low concentrations only and has been enriched by affinity chromatography. Glial cells of human brain were separated from neurons by a density centrifugation method and four fractions were obtained: neuropil, neuronal perikarya, nuclei and debris. Each fraction was checked by light and phase contrast microscopy to estimate the intactness of the cells and any contamination by other fractions, and also immunologically for determination of brain‐specific alpha2‐glyco‐protein. The results indicate that localization of this brain‐specific protein is in the cyto‐plasm of the glial cells. The results are discussed in terms of a possible role of this protein in the inflammatory response and in some demyelinating diseases.
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Warecka et al. (1972) studied this question.
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