Evaluation of Isolated Helicobacter Pylori genes from dental plaques and association with gastrointestinal ulcers by multiplex PCR method

Background & Aim: Helicobacter pylori is a major cause of gastric ulcer and peptic ulcer and is considered a risk factor for gastric cancer.Gingival grooves in the teeth of people with chronic periodontitis may act as a reservoir for H. pylori.Therefore, the aim of this study was to investigate the virulence genes of H. pylori isolated from dental plaques. Material & Methods:The study was cross--sectional and from 120 patients with periodontitis referring to Tehran dental clinics, 111 dental plaque samples were isolated.Sterile physiology serum was used to transfer the sample.Growth in Brucella agar + 1% starch + 10-7% at 37 ° C and 95% humidity and CO2 concentration of 0.5% in CO2 incubator.In order to identify the molecules, DNA extraction was performed and then the sequences of JW22 and JW23 regions were used for accurate identification.Results: Of the 111 dental plaque samples taken from 120 participants in this study, 22 strains (19%) were positive for H. pylori which 10 strains carrying different H. pylori virulence genes.The molecular analysis of the studied genes showed that the frequency of cagA, cagT, cagE, vacA and hrgA genes showed that the prevalence of these genes was 19% of H. pylori strains of 3 (57.5%), 1 (0.19%), %), 2 (0.34%), 1 (19.1%) and 1 (0.19%), respectively.Conclusions: It seems that in spite of the presence of Helicobacter pylori virulence genes in the dental plaque, to control the related diseases in addition to antibiotic treatments periodontal treatments and dental plaque control are also required.