Summary Cholesterol purifiedvia the dibromide has been compared with two commercial preparations for use as standard materials for assay of cholesterol in biological materials. It is concluded that determination of the specific extinction coefficient, following application of the Liebermann‐Burchard reaction under specified conditions, is sufficient to ascertain adequacy of a sample of cholesterol for use as a standard. Autoxidation of cholesterol by aeration in colloidal aqueous suspension resulted in losses of assay of cholesterol by the Schoenheimer‐Sperry procedure which were roughly equal in amount to the percentage of conversion of cholesterol to diols and ketone. Assay of the same material by the Liebermann‐Burchard reaction alone resulted in no apparent losses through autoxidation. Cholesterol was not autoxidized by refluxing in ethanol‐ether in the presence of corn oil.
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L. N. Norcia (1958) studied this question.
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