The Amino Acid Sequence of Cytochrome c from Debaryomyces kloeckeri*

The amino acid sequence of cytochrome c from Debaryomyces kloeckeri, one of the yeast species, was elucidated in order to find out structural similarity or dissimilarity comparing with those from different species of yeast. Structures of the peptides in the tryptic digest of the cytochrome were determined and the information on the arrangement of the tryptic peptides was obtained from the chymotryptic peptides. The complete amino acid sequence of Debaryomyces cytochrome c containing 109 residues was elucidated. The number of the amino acid residues and the primary structure of the Debaryomyces protein resembled that of Candida krusei rather than that of Saccharomyces oviformis containing 108 residues. The 78th lysine (the 72nd starting from the common glycine in all mammalian-type cytochromes c) from the N-terminus was trimethylated as in other cytochromes c from Candida, Saccharomyces, Neurospora and some plants.