Key result
A novel heparinoid polymer, poly(NVA-co-VAm-co-VSA), prolonged human whole blood clotting time and APTT compared with poly(NVA) and poly(NVA-co-VAm), with activity increasing with N-sulfate groups.
Why the study?
Does poly(NVA-co-VAm-co-VSA) improve anticoagulant properties in human whole blood compared to poly(NVA) and poly(NVA-co-VAm)?
Does poly(NVA-co-VAm-co-VSA) improve anticoagulant properties in human whole blood compared to poly(NVA) and poly(NVA-co-VAm)?
The addition of N-sulfate components to synthetic polymers endows them with anticoagulant activity, as demonstrated by prolonged clotting times in vitro.
May guide synthetic anticoagulant polymer design; leaves open in vivo efficacy and clinical translation.
A novel heparinoid polymer was prepared with an N-sulfate side chain group by sulfation of poly( N-vinylacetamide-co-vinylamine) [poly(NVA-co-VAm)] which was obtained by the partial hydrolysis of poly( N-vinylacetamide) [poly(NVA)]. The hydrolysis of poly(NVA) was controlled by reaction time and temperature and the sulfation of poly(NVA-co-VAm) was performed with chlorosulfonic acid. Poly( N-vinylacetamide-co-vinylamine-co-vinylsulfamic acid) [poly(NVA-co-VAm-co-VSA)] was confirmed by means of IR spectroscopy, and the degree of sulfation of poly(NVA-co-VAm-co-VSA) was estimated by elemental analysis. The anticoagulant properties of poly(NVA-co-VAm-co-VSA) were evaluated using the Lee-White clotting test and activated partial thromboplastin time (APTT) test. The human whole blood clotting time in the presence of poly(NVA-co-VAm-co-VSA) was prolonged compared with poly(NVA) and poly(NVA-co-VAm). The clotting time was prolonged with increased N-sulfate groups on the polymer. APTT tests of the polymer were also prolonged with N-sulfate increases. The results suggest that the N-sulfate components on the polymer can endow anticoagulant activity to synthetic polymers.
No takes yet. Share an insight, caveat, or question.
Sakamoto et al. (1999) studied this question. poly(NVA-co-VAm-co-VSA) vs. poly(NVA) and poly(NVA-co-VAm) was evaluated on Anticoagulant properties (Lee-White clotting test and APTT). A novel heparinoid polymer, poly(NVA-co-VAm-co-VSA), prolonged human whole blood clotting time and APTT compared with poly(NVA) and poly(NVA-co-VAm), with activity increasing with N-sulfate groups.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: