Glutamine is shown to be the effector compound for the regulation of both nitrogenase and glutamine synthetase activities in Rhodopseudomonas sphaeroides f. sp. denitrificans. Glutamine synthetase and nitrogenase (components I and II) in this bacterium have similar properties to those from Rhodopseudomonas capsulata. Ammonia-shock treatment of washed cells, which resulted in an accumulation of [14C]glutamine from [14C]glutamate, was accompanied by an adenylylation of glutamine synthetase and an inhibition of nitrogenase. Treatment of cells with azaserine (which inhibits glutamate synthase) enhanced the accumulation of labelled glutamine from [14C]glutamate and 15NH4C1. Glutamine inhibited the nitrogenase activity of azaserine-treated cells to a greater extent than that of untreated cells.
No takes yet. Share an insight, caveat, or question.
Michalski et al. (1984) studied this question.
Synapse has enriched one closely related paper. Consider it for comparative context: