Key result
Humanin administration before myocardial infarction in rats significantly reduced myocardial injury markers and tissue damage, with cardioprotective effects mediated via the Notch signaling pathway.
Why the study?
The study investigated the protective role of humanin against myocardial injury when applied before myocardial infarction, as well as its cellular mechanism involving the Notch pathway.
Does humanin pretreatment prevent myocardial injury in an isoproterenol-induced myocardial infarction rat model?
Does humanin pretreatment prevent myocardial injury in an isoproterenol-induced myocardial infarction rat model?
Humanin pretreatment exerts cardioprotective effects in a rat model of myocardial infarction, likely mediated via activation of the Notch signaling pathway.
May attenuate rat MI injury via Notch; leaves open human translation.
Background/aim: By applying humanin (HN) before myocardial infarction (MI), its protection in myocardial injury and the possible roles of its cellular mechanism in the Notch pathway were investigated. Materials and methods: The study was carried out at Fırat University Experimental Research Center (12/24/2018-12/23/2019). Spraque-Dawley rats were divided into 10 groups: I (control) (n = 6), II (HN 6 h) (n = 6), III (HN 24 h) (n = 6), IV (HN day 7) (n = 6), V (MI 6 h) (n = 7), VI (MI 24 h) (n = 7), VII (MI day 7) (n = 7), VIII (MI+HN 6 h) (n = 7), IX (MI+HN 24 h) (n = 7), and X (MI+HN day 7) (n = 7). To create MI, 200 mg/kg of isoproterenol (ISO) was administered to the rats subcutaneously. Moreover, 252 μg/kg of HN was given intraperitoneally (ip) to the rats on its own and before MI. Molecular parameters Notch1, Notch2, Hes1, Hes2, Jagged1, Jagged2, DLL1, and DLL4 were examined using polymerase chain reaction in the heart tissue, Notch1, Hes1, and DLL4 were examined using western blot, while heart tissue was taken for histochemical examinations. Results: The mRNA expression levels of the Notch signaling members (Notch1, Notch2, Hes1, Hes2, Jagged1, Jagged2, DLL1, and DLL4) tended to decrease after MI. The Notch signaling members increased more significantly, especially toward day 7 after HN application before MI. In the western blot anylyses, the Notch1, Hes1, and DLL4 protein levels increased significantly toward day 7 in the groups given HN before MI. Moreover, the serum AST, LDH, CK-MB, and troponin I levels tended to decrease with the application of HN before MI and there was a significant decrease in edema, hemorrhage, and mononuclear cells in the heart tissue at 24 h post-MI and fibrosis on day 7 post-MI. Conclusion: HN administration before MI has a cardioprotective effect on rats via the Notch signaling pathway.
No takes yet. Share an insight, caveat, or question.
Onat et al. (2023) studied Myocardial infarction (n=66). Humanin vs. Isoproterenol-induced MI without humanin was evaluated on Notch signaling pathway activation and myocardial injury markers (AST, LDH, CK-MB, Troponin I) (p=<0.05). Humanin administration before myocardial infarction in rats significantly reduced myocardial injury markers and tissue damage, with cardioprotective effects mediated via the Notch signaling pathway.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: