What is the clinical evidence from this study?

Study design: Other. Intervention: WAVE2 inhibition vs. Control. Primary outcome: F-actin-rich membrane protrusions and macrophage migration.

November 9, 2005Journal of Cell Science

A WAVE2-Abi1 complex mediates CSF-1-induced F-actin-rich membrane protrusions and migration in macrophages

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Key result

Inhibition of WAVE2 or Abi1 in macrophages significantly reduced CSF-1-elicited F-actin protrusions and impaired cell migration.

Population

Primary macrophages and cells derived from the murine monocyte/macrophage RAW264.7 cell line (RAW/LR5)

Comparison

Inhibition of WAVE2 function and targeting Abi1 vs Uninhibited cells

Design

Preclinical

Authors

WAWassim Abou‐KheirAmerican University of Beirut JGJean-Claude GevreyAlbert Einstein College of Medicine HYHideki YamaguchiSasaki Institute

Discussion

Member takes

Implication

WAVE2-Abi1 inhibition impairs macrophage migration in animal models; leaves open relevance to human cardiovascular inflammation.

Structured PICO

Population

Primary macrophages and cells derived from the murine monocyte/macrophage RAW264.7 cell line (RAW/LR5)

Intervention

Inhibition of WAVE2 function (via dominant-negative mutant, anti-WAVE2 antibodies, or RNAi) and targeting Abi1

Comparator

Uninhibited cells (resting or stimulated with CSF-1)

Outcome

Formation of F-actin-rich membrane protrusions and cell migration in response to CSF-1surrogate

The WAVE2-Abi1 complex is crucial for normal actin cytoskeleton reorganization and migration of macrophages in response to CSF-1.

Cite This Study

Abou‐Kheir et al. (2005) studied this question. WAVE2 inhibition vs. Control was evaluated on F-actin-rich membrane protrusions and macrophage migration. Inhibition of WAVE2 or Abi1 in macrophages significantly reduced CSF-1-elicited F-actin protrusions and impaired cell migration.

synapsesocial.com/papers/6a8b09b9c716e89f35ded0de https://doi.org/10.1242/jcs.02638

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November 9, 2005Journal of Cell Science

A WAVE2-Abi1 complex mediates CSF-1-induced F-actin-rich membrane protrusions and migration in macrophages

View Full Paper

Ask AI

Bookmark

Key result

Inhibition of WAVE2 or Abi1 in macrophages significantly reduced CSF-1-elicited F-actin protrusions and impaired cell migration.

Population

Primary macrophages and cells derived from the murine monocyte/macrophage RAW264.7 cell line (RAW/LR5)

Comparison

Inhibition of WAVE2 function and targeting Abi1 vs Uninhibited cells

Design

Preclinical

Authors

WAWassim Abou‐KheirAmerican University of Beirut JGJean-Claude GevreyAlbert Einstein College of Medicine HYHideki YamaguchiSasaki Institute

Discussion

Member takes

Implication

WAVE2-Abi1 inhibition impairs macrophage migration in animal models; leaves open relevance to human cardiovascular inflammation.

Structured PICO

Population

Primary macrophages and cells derived from the murine monocyte/macrophage RAW264.7 cell line (RAW/LR5)

Intervention

Inhibition of WAVE2 function (via dominant-negative mutant, anti-WAVE2 antibodies, or RNAi) and targeting Abi1

Comparator

Uninhibited cells (resting or stimulated with CSF-1)

Outcome

Formation of F-actin-rich membrane protrusions and cell migration in response to CSF-1surrogate

The WAVE2-Abi1 complex is crucial for normal actin cytoskeleton reorganization and migration of macrophages in response to CSF-1.

Cite This Study

synapsesocial.com/papers/6a8b09b9c716e89f35ded0de https://doi.org/10.1242/jcs.02638

Also Consider

Synapse has enriched 2 closely related papers on similar clinical questions. Consider them for comparative context:

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