Scaffold is one of the 3 main components in the field of tissue engineering and regenerative medicine. Fabrication technology is widely used to produce temporary scaffold from biomaterials, either synthetic or natural. Application of implantation technique preferably demands natural biomaterial due to their excellent biocompatibility and biodegradability, as well as low antigenicity. Besides, due to their biomechanical properties, natural biomaterials play a vital role in tissue engineering to provide the suitable strength, modulus, and resistance.1 This study aimed to extract collagen from ovine tendon, and evaluate its genotoxic and cytotoxic effects on human dermal fibroblast cells (HDFCs) considering the use in skin tissue engineering. All experimentations in this study were performed from June 2009 to April 2010. This study has been approved by the Ethical Committee of Universiti Kebangsaan Malaysia. Skin samples were obtained from 6 consenting patients as redundant tissue after surgery. The HDFCs were isolated and suspended in equivalent mixture of Ham’s F-12: high glucose Dulbecco’s
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Chowdhury et al. (2011) studied this question.