Characterization and Intragenic Position of Mutations in the Gene for Galactoside Permease of Escherichia coli

To test certain models for the structure of the gene for galactoside permease and its product, appropriate mutants of E. coli were isolated after mutagenic treatment, partially characterized and mapped. The 69 mutants found to be in the permease gene were mapped by crosses with episomal deletions which divided the gene into 13 regions.