STUDY of the role of the pituitary-adrenal axis in protein metabolism is desirable in view of the conflicting reports of Novack (1952) and of White et al. (1948), Friedberg (1950) and Marshall and Friedberg (1951). Novack showed the administration of adrenocorticotrophin (ACTH) to increase C14-histidine incorporation into the visceral and muscle protein of the intact mouse. The other authors, in studies employing C14-glycine or S35-methionine, showed adrenalectomy to increase incorporation into the tissues of the fasted mouse and the administration of dehydrocorticosterone to the adrenalectomized rats or ACTH to the hypophysectomized rat to suppress amino acid incorporation. In an attempt to resolve this conflict we have studied the role of the pituitary-adrenal axis in the incorporation of S35-cystine into tissue proteins of the albino rat. methods Equal numbers of male and female Sprague-Dawley rats were used in all experiments and were kept on an ad libitum regime of Purina diet at all times.
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Lee et al. (1952) studied this question.