Unprimed Synthesis of Polyadenylate and Polyuridylate Catalyzed by Ribonucleic Acid Polymerase

When adenosine triphosphate and uridine triphosphate are incubated with ribonucleic acid polymerase in the absence of a template, polyadenylate and polyuridylate are formed. The reaction occurs after a lag period of variable length. Unprimed synthesis of polyadenylate and polyuridylate can be greatly inhibited through the use of sufficient amounts of templates of high molecular weight.