Interactions between Fraction 1 leaf protein and other surfactants involved in the bloat syndrome

Polygalacturonic acid and salivary macromolecules increased the persistence of Fraction 1 leaf protein foams generated in vitro . The increase in persistence effected by polygalacturonic acid could be explained by increased bulk viscosity which decreased the foam drainage of the mixed foam. The effect of the salivary molecules, i.e., bovine salivary mucoprotein and oesophageal mucin, could not totally be explained by viscosity. A study of the reaction between salivary mucoprotein and Fraction 1 protein indicated a specific, non‐ionic, pH‐dependent interaction between the molecules at surfaces only and not in solution.