The interaction of rat liver ribosomal proteins L6, L8, L19, S9, and 513 with 5.8 S rRNA was characterized by nitrocellulose membrane filtration.Binding approached saturation with the five proteins; the apparent association constants (IC'=), measured at 4 "C and 22 "C, ranged from 0.2 to 18 X los M-'.The molar ratio ' The pH of solutions and buffers was determined at 20 "C.5 S rRNA may be close to 5.8 S rRNA in the 60 S subunit 14.
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Todokoro et al. (1981) studied this question.
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