Exponentially growing yeast cells showed a drastic shrinkage when irradiated in water suspension (but not in buffer) with broad‐band near‐UV radiation. This was clearly seen soon after irradiation in size‐distribution curves measured by a Coulter counter. This early shrinkage was observed at a near‐UV fluence where the survival enters a region of exponential decline after a large shoulder. We further observed the formation of a distinct band in a density‐gradient‐centrifugation profile, presumably corresponding to the near‐UV‐affected cells. A survival test showed that the cells making up this band were dead.
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Watanabe et al. (1982) studied this question.
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