Interspecific hybridization between Nicotiana trigonophylla Dun. and N. tabacum L. through ovule culture.

Interspecific hybrids between N. trigonophylla (2n=24, ♀) and N. tabacum (2n=48, ♂) were obtained through in vitro culture of hybrid ovules. Hybrid plants were produced efficiently, when the medium conditions were suitable. All of the hybrid plants were morphologically uniform, cytologically stable and completely selfsterile. The pollen viabilities of the amphidiploid plants induced by colchicine treatments exceeded 90%, while viable seeds were obtained only from about 5% of the ovules.