The effects of self-etch bio-active dental adhesive systems on bacterial microleakage

The aim of this in vitro study was to evaluate the effects of bio-active and non-bio-active self-etch adhesive sytems on bacterial microleakage of class II box cavities with restoration margins in dentin. Two bio-active (Clearfil Protect Bond and F1 Bond II) and two nonbio-active (Clearfil SE Bond and Clearfil s3 Bond) adhesive systems were selected for the study. Eighty mesial and distal class II box cavities were prepared on fourty sound human third molar teeth. The teeth were then divided into four groups with 10 teeth in each group. Groups were restorated using different adhesive systems. The restored specimens were immersed into a culture medium containing streptococcus mutans for bacterial microleakage test. Serial sections of the specimens were used to evaluate the bacterial lekage by using light microscope. The data obtained from the test of bacterial microleakage was evaluated using 2-way mixed analysis of variance (p < 0.05). Statistical analysis showed significant differences between the groups (p < 0.05). Nevertheless it was observed that none of the tested adhesive systems including the bio-active ones were successfull in eliminating bacterial microleakage.