October 26, 2016Scientific ReportsOpen Access

Super-resolution imaging of fluorescently labeled, endogenous RNA Polymerase II in living cells with CRISPR/Cas9-mediated gene editing

View Full Paper

Ask AI

Bookmark

Population

Mouse embryonic fibroblast (MEF) cells

Comparison

CRISPR/Cas9-mediated gene editing to label… vs Previous exogenous Pol II MEF cell line

Design

Preclinical

Authors

WCWon‐Ki ChoKorea Advanced Institute of Science and Technology NJNamrata JayanthTata Institute of Fundamental Research SMSusan MullenPlanetary Science Institute

Discussion

Member takes

Implication

Validates exogenous Pol II models in animals; confirms prior dynamics and leaves open human cardiovascular applications.

Structured PICO

Population

Mouse embryonic fibroblast (MEF) cells

Intervention

CRISPR/Cas9-mediated gene editing to label endogenous RNA Polymerase II

Comparator

Previous exogenous Pol II MEF cell line (historical comparison)

Outcome

Pol II clustering dynamics (cluster lifetime, response to serum-stimulation and drug-mediated transcription inhibition)surrogate

CRISPR/Cas9-mediated labeling of endogenous RNA Polymerase II confirms that previous exogenously tagged Pol II faithfully recapitulated endogenous polymerase clustering dynamics in living cells.

Cite This Study

Cho et al. (2016) studied this question.

synapsesocial.com/papers/6a8bc719f07ad6ed43b22b43 https://doi.org/10.1038/srep35949

View Full Paper

Ask AI

Bookmark

Super-resolution imaging of fluorescently labeled, endogenous RNA Polymerase II in living cells with CRISPR/Cas9-mediated gene editing

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Super-resolution imaging of fluorescently labeled, endogenous RNA Polymerase II in living cells with CRISPR/Cas9-mediated gene editing

Discussion

Member takes

Implication

Structured PICO

Cite This Study

Also Consider

Also Consider