A cationic block copolymer (mPEG‐b‐PMETAC) with uniform molecular weight was synthesized using atom transfer radical polymerization. Insulin was reversibly encapsulated by mPEG‐b‐PMETAC via electrostatic interaction. The secondary and tertiary structures of insulin during the assembly and delivery processes were monitored by circular dichroism and fluorescence spectrum. The effects of pH and salt concentration on encapsulation were examined, respectively. Enhanced stability of the encapsulated insulin against proteolysis by trypsin and chymotrypsin was demonstrated. Insulin can be encapsulated and delivered from an mPEG‐b‐PMETAC assembly by tuning the pH and ionic strength, which determines the electrostatic interaction between insulin and the polymer.
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Li et al. (2015) studied this question.
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