Enzyme preparations that are stable in organic solvents and in aqueous media can be obtained by covalently cross-linking enzyme crystals. The resulting exceptionally stable biocatalysts, the “cross-linked enzyme crystals” (CLECs), efficiently catalyze enzymatic peptide syntheses (see example below), esterfications, hydrolyses, CC bond-forming reactions, and reductions. Z = benzyloxycarbonyl.
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Zelinski et al. (1997) studied this question.
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