A simple radiometric method is described to determine the incorporation of acetate or pyruvate into citrate. Following in vitro incorporation of these substrates by the tissues, they are converted to ‘active acetate' which then condenses with oxaloacetate to form citrate. When 1-14C acetate or 2-14C pyruvate were added as tracers, the citrate was labeled at 1-14C carboxyl position which on oxidation was released as 14CO2. Labeled acetate in the medium did not interfere with the quantitative recovery of small quantities of citrate formed. When monofluoroacetate (MFA) instead of acetate was added with labeled acetate as the substrate, monofluorocitrate was formed which accumulated in the tissues, perhaps due to block in further oxidation. There was an eight-fold increase in the labeled CO2 evolution by liver tissue when MFA was used as the substrate.
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Dixit et al. (1974) studied this question.