What question did this study set out to answer?

To describe and evaluate an enzymatic cycling method for measuring low concentrations of pyridine nucleotides using enzymatic dismutation.

October 1, 1961Journal of Biological ChemistryOpen Access

The Measurement of Pyridine Nucleotides by Enzymatic Cycling

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Authors

OLOliver H. LowryWashington University in St. Louis JPJanet V. PassonneauNational Institute on Alcohol Abuse and Alcoholism DSDemoy W. SchulzUniversity of Tübingen

Discussion

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Implication

Methodological analysis reveals linear amplification ranges for pyridine nucleotide quantification, highlighting distinct cycling dynamics between TPN and DPN systems.

Key Points

To describe and evaluate an enzymatic cycling method for measuring low concentrations of pyridine nucleotides using enzymatic dismutation.
Target coenzymes were used to catalyze enzymatic dismutation between two substrates over several thousand cycles at concentrations well below their Michaelis constants.
DPN+ standards scaling in two-fold increments, along with parallel blanks, were analyzed alongside samples to account for non-linear pyruvate formation.
Reaction rates remained proportional to nucleotide concentrations when nucleotides were kept far below their Michaelis constants.
The TPN cycling system maintained proportionality across a 100-fold range of coenzyme concentrations using the same reagent.
The DPN cycling system demonstrated near-linear proportionality across a narrower 20-fold range due to rate deceleration from pyruvate accumulation.

Cite This Study

Lowry et al. (1961) studied this question.

synapsesocial.com/papers/6a8bec12e066a6a97ec45e0a https://doi.org/10.1016/s0021-9258(19)61729-1

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October 1, 1961Journal of Biological ChemistryOpen Access

The Measurement of Pyridine Nucleotides by Enzymatic Cycling

View Full Paper

Ask AI

Bookmark

Authors

OLOliver H. LowryWashington University in St. Louis JPJanet V. PassonneauNational Institute on Alcohol Abuse and Alcoholism DSDemoy W. SchulzUniversity of Tübingen

Discussion

Member takes

Implication

Methodological analysis reveals linear amplification ranges for pyridine nucleotide quantification, highlighting distinct cycling dynamics between TPN and DPN systems.

Key Points

To describe and evaluate an enzymatic cycling method for measuring low concentrations of pyridine nucleotides using enzymatic dismutation.
Target coenzymes were used to catalyze enzymatic dismutation between two substrates over several thousand cycles at concentrations well below their Michaelis constants.
DPN+ standards scaling in two-fold increments, along with parallel blanks, were analyzed alongside samples to account for non-linear pyruvate formation.
Reaction rates remained proportional to nucleotide concentrations when nucleotides were kept far below their Michaelis constants.
The TPN cycling system maintained proportionality across a 100-fold range of coenzyme concentrations using the same reagent.
The DPN cycling system demonstrated near-linear proportionality across a narrower 20-fold range due to rate deceleration from pyruvate accumulation.

Cite This Study

Lowry et al. (1961) studied this question.

synapsesocial.com/papers/6a8bec12e066a6a97ec45e0a https://doi.org/10.1016/s0021-9258(19)61729-1

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