Assignment and analysis of the 500 MHz 1H NMR spectra of somatostatin and the acyclic precursor (S3,14‐Acm)‐somatostatin in dimethyl sulphoxide

The 500 MHz 1H NMR spectra of somatostatin and the precursor, di‐S3,14‐acetamidomethyl dihydrosomatostatin, in dimethyl sulphoxide solution have been assigned. Chemical shifts, coupling constants and NH shifts vs temperature coefficients are tabulated. The data are consistent with a stable β‐turn/β‐sheet conformation for the precurso, while somatostatin itself appears to be more conformationally mobile, with an average conformation significantly different from that in aqueous solution.