Answers are grounded in this paper and cite their sources.
Activation of the Epstein-Barr virus (EBV) genome by 5-bromodeoxyuridine (BUDR) in “virus-free” Raji or NC37 cells resulted in the synthesis of early viral antigens (EA) detectable by immunofluorescence. A high proportion of sera (65-97%) from patients with infectious mononucleosis, Burkitt lymphoma and nasopharyngeal carcinoma reacted with these antigens while only 1/3 of the sera from healthy persons were reactive. Forty to 80% of sera from subhuman primates also contained anti-EA antibodies. The significance of these antibodies in man in relation to EBV infection or associated illnesses remains obscure. EA appeared to be immunologically distinct from EBV-related soluble complement-fixing antigens. BUDR activation of Raji or NC37 cells offers a simple and convenient method of obtaining EA preparations free of viral capsid antigens.
No takes yet. Share an insight, caveat, or question.
Gerber et al. (1972) studied this question.
Synapse has enriched 10 related papers. Consider them for comparative context: