Suspensions of isolated adrenal cells respond to ACTH with increased steroidogenesis. This isolated adrenal cell system has the prerequisite sensitivity, accuracy, and specificity for the determination of ACTH when coupled with Quso processing of serum. Quso mixed with serum adsorbs the ACTH therein; 0.1 N HC1 quantitatively elutes the ACTH from the Quso. Substances in serum which interfere with the assay of ACTH in the isolated adrenal cell system are eliminated.
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Giordano et al. (1971) studied this question.