Methodological protocol demonstrates high-throughput agar dilution screening across 384 bacterial strains, suggesting improved efficiency for large-scale antibiotic susceptibility testing.
Conventional minimum inhibitory concentration (MIC) assays used to determine the antibiotic susceptibility of microorganisms are inherently low-throughput and labor intensive. Here, we present a high-throughput agar dilution MIC assay to efficiently assess resistance profiles across large collections of bacterial isolates. We describe steps for agar plate preparation, culture spotting, imaging setup, and semi-automated image-based data analysis of this high-throughput assay. This approach enables simultaneous determination of antibiotic resistance levels for 384 strains across multiple antibiotics within a single workflow. For complete details on the use and execution of this protocol, please refer to Van den Bergh et al. 1
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Agten et al. (2026) studied this question.
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