A facile procedure for the incorporation of Ru(bpy) 3 2+ in an oligonucleotide is reported. A Ru(bpy) 3 2+ phosphoramidite is synthesized, and then attached to the 5‘-terminus of DNA using a standard protocol on an automated DNA solid-phase synthesizer. Photophysical studies of the Ru(II) tris-diimine complex as well as the corresponding labeled oligonucleotides demonstrate that the excited-state electron is localized on one specific bipyridine with the dipole directed toward the linkage to DNA, and that the Ru(II) excited state is long-lived when attached to the DNA.
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Khan et al. (1999) studied this question.
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