Purified histone fractions were found to interact with the mitochondrial membrane system, causing a reduction in optical density as a result of swelling and aggregation. The arginine-rich f2a was the most active fraction; threshold concentration was approximately 1 µg per mg of mitochondrial protein. Adenosine 5‘-triphosphate slowed and guanosine 5‘-triphosphate prevented the histone-induced swelling, while all other nucleoside mono-, di-, and triphosphates were without effect. Oligomycin, aurovertin, or strontium enhanced the protective action of ATP. Amytal, azide, antimycin, 2,4-dinitrophenol, and carbonyl cyanide m-chlorophenylhydrazone did not significantly affect the histone-induced changes. Octylguanidine and chlorpromazine markedly retarded the swelling process. The most active polyanionic protective compounds were gangliosides, phosphoproteins, and phosphatidylserine.
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Schwartz et al. (1966) studied this question.
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