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A study of the effects of β-ecdysone on the initiation of cuticle deposition and seta formation by cockroach leg regenerates in vitro showed that both processes are ecdysone-dependent and are initiated by the same threshold dose, but the responses differ qualitatively and quantitatively. The initiation of cuticle deposition depends primarily on the accumulation of hormone-initiated events by the target tissue. The initiation of seta formation has an additional requirement for simultaneous protein synthesis.
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Edwin P. Marks (1973) studied this question.
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