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A gas chromatographic procedure for the quantitative analysis of the inhalation anesthetics in blood was devised. A blood sample was equilibrated in 10 ml. or 20 ml. glass vial. After equilibration 1 ml. of the gas in the vial was introduced into a gas chromatograph and the anesthetic concentration in blood was measured from that in gas phase by means of a calibration curve prepared from appropriate standards. A thermal conductivity detector was used for the analysis of nitrous oxide, cyclopropane and ether, while a flame ionization detector was used for halothane, methoxyflurane and low concentration of ether. Concentrations in blood of inhalation anesthetics now available can be measured accurately by this method.
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Yamamura et al. (1966) studied this question.
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