A chitosan (CS)‐tin oxide (SnO2) nanobiocomposite film has been deposited onto an indium‐tin‐oxide glass plate to immobilize cholesterol oxidase (ChOx) for cholesterol detection. The value of the Michaelis–Menten constant (Km) obtained as 3.8 mM for ChOx/CS‐SnO2/ITO is lower (8 mM) than that of a ChOx/CS/ITO bioelectrode revealing enhancement in affinity and/or activity of ChOx towards cholesterol and also revealing strong binding of ChOx onto CS‐SnO2/ITO electrode. This ChOx/CS‐SnO2/ITO cholesterol sensor retains 95% of enzyme activity after 4–6 weeks at 4 °C with response time of 5 s, sensitivity of 34.7 μA/mg dL−1 cm2 and detection limit of 5 mg/dL.
No takes yet. Share an insight, caveat, or question.
Ansari et al. (2009) studied this question.
Synapse has enriched 3 closely related papers on similar clinical questions. Consider them for comparative context: