An actin preparation extracted at 25 degree C for 2 h from the residue which had previously been subjected to the routine extraction procedure, i.e. at 4 degree C for 15 min, showed markedly increased rates of amino acid incorporation, i.e. 48% higher than routinely extracted actin. The nature of the actin having higher amino acid incorporation rates was discussed in relation to 10S-actinin.
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Kazuhiro Kohama (1980) studied this question.