Key result
Mn2+-Phos-Tag Polyacrylamide gel technology successfully resolves phosphorylated proteins extracted from cardiac and skeletal muscles to accelerate phosphoproteomics research.
This protocol provides a method for separating phosphorylated proteins using Phos-tag gel technology, which can accelerate phosphoproteomics research in cardiac and other tissues.
Lacks immediate clinical application; extends basic science methodology by providing a reliable tool.
Phos-tag gel technology to separate phosphorylated proteins from their unphosphorylated counterparts. It provides key insights into methods for careful sample preparation and experimental directions for determining the appropriate Phos-tag gel compositions and electrophoresis and western blotting conditions. This protocol has been used to successfully resolve proteins extracted from cardiac and skeletal muscles. The guidelines can be extended for optimizing protocols to resolve proteins from other cells or tissue sources. With this, phosphoproteomics and the elucidation of underlying mechanisms of disease progression can be accelerated.
No takes yet. Share an insight, caveat, or question.
Markandran et al. (2021) studied this question. Mn2+-Phos-Tag Polyacrylamide gel electrophoresis was evaluated on Separation of phosphorylated proteins from unphosphorylated counterparts. Mn2+-Phos-Tag Polyacrylamide gel technology successfully resolves phosphorylated proteins extracted from cardiac and skeletal muscles to accelerate phosphoproteomics research.
Synapse has enriched 5 closely related papers on similar clinical questions. Consider them for comparative context: