Tracing method study of bacterial translocationin vivo

Endogenesis infection plays an important role in nosocomial infection[1-3]. By studying progress of bacteria translocation from intestinal tract, the concept of gut origin infection has been accepted gradually[4-6]. Because of no ideal tracing method, there were some controversies. In order to solve the problem, the PUC19 plasmid vector tracing method with restriction map analysis and fluorescence labeling method were used to study gut-origin bacterial translocation. According to the characteristic of PUC19 plasmid, a special animal model was designed and two methods were compared.