Two hundred four examples of blood group antibodies, reactive only by the indirect antiglobulin test, were examined by the two-layer albumin capillary (TLAC) technique. Fifty-nine per cent of the examples tested were reactive by this technique. TLAC reactivity appeared to be related to antibody specificity, but a positive association between TLAC reactivity and IgG subclass composition was not confirmed.
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Hardman et al. (1981) studied this question.