Temperature-Sensitive Mutants of Bacteriophage T4d: Their Isolation and Genetic Characterization

R. S. Edgar; I Lielausis

doi:10.1093/genetics/49.4.649

April 1, 1964Genetics

Temperature-Sensitive Mutants of Bacteriophage T4d: Their Isolation and Genetic Characterization

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Key Points

To isolate and characterize temperature-sensitive conditional lethal mutants of bacteriophage T4D to identify and map genes essential for viral growth.
Mutagenized bacteriophage T4D stocks and screened plaques for growth at permissive (25–30°C) versus nonpermissive (40–42°C) temperatures.
Conducted complementation assays and two-factor genetic crosses to determine functional gene groups and chromosomal map positions.
Successfully identified numerous distinct temperature-sensitive mutants distributed across multiple functional complementation groups throughout the T4D genome.
Demonstrated that conditional lethal temperature-sensitive mutations enable comprehensive genetic mapping of essential viral functions previously inaccessible by standard mutant selection.

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Implication

Experimental study demonstrates isolation and genetic mapping of temperature-sensitive mutants in bacteriophage T4D, revealing essential viral gene functions.

Abstract

R S Edgar, I Lielausis; TEMPERATURE-SENSITIVE MUTANTS OF BACTERIOPHAGE T4D: THEIR ISOLATION AND GENETIC CHARACTERIZATION, Genetics, Volume 49, Issue 4, 1 A

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Edgar et al. (1964) studied this question.

synapsesocial.com/papers/6a8c2a89f07ad6ed43b252e5 https://doi.org/10.1093/genetics/49.4.649

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Temperature-Sensitive Mutants of Bacteriophage T4d: Their Isolation and Genetic Characterization

Key Points

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Temperature-Sensitive Mutants of Bacteriophage T4d: Their Isolation and Genetic Characterization

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Abstract

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