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Controlling alginate gel formation by diffusion of Ca(2+) ions through a filter barrier, a layer-by-layer deposition technique with resolution on the size scale of a single cell is presented. It offers the possibility of exposing cells under biocompatible conditions to microheterogeneous three-dimensional environments, mimicking the layered structure of extracellular matrix in tissues.
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Braschler et al. (2010) studied this question.
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