The metabolism of 2-fluoroethanol

Methods of preparation of three distinct fractions of histones have been described and applied to the thymus, spleen and liver of calves and to the liver and spleen of normal and leukaemic rats.2. No differences in amino acid composition or in N-terminal groups of the same fractions from the different sources were found, outside the probable experimental errors.On the other hand the char- acteristic analytical differences between the fractions were apparent in all the preparations.3. The purity of the fractions was examined by starch-gel electrophoresis.Apart from minor bands observed in some cases and probably due to un- removed impurities, no differences of the patterns or of the mobilities were observed which could be ascribed to tissue or species specificity.4. Some qualification of the above statements are necessary for fractions derived from the Ehrlich ascites tumour.A complete resolution into the fractions was not achieved in this case.We are indebted to the World Health Organization for the award of a Research Fellowship to one of us (L.H.) and to Dr V. Holoubek who made available the histones from leukaemic rat spleen and liver which he had prepared.We also wish to thank Dr D. M. P. Phillips for useful dis- cussions.