Differential gene expression between in vivo and in vitro maturation: a comparative study with bovine oocytes derived from the same donor pool

OBJECTIVE: obtained from the same group of donors. Transcript abundance in oocytes from the IN VIVO-OPU group and oocytes matured in vitro but retrieved from different cows slaughtered at a commercial abattoir (IN VITRO-Abattoir group) was also compared. Total RNA was extracted from denuded oocytes and cDNA was produced via reverse transcription using an oligo(dT) primer for relative quantification of eight target transcripts by real-time PCR. CONCLUSION: maturation induced by exogenous hormones in oocytes collected from the same donor pool.