Effect of diffusion on excitation energy transfer and migration in a dye pair sodium fluorescein (donor) and Rhodamine-6G (acceptor) has been studied for different viscosities by both steady state and time domain fluorescence spectroscopic measurements. The donor-donor interaction appears to be weaker as compared to donor-acceptor interaction and thus favors direct Forster-type energy transfer. Interestingly, at low viscosity (water in this case) transfer appears to be controlled by material diffusion/energy migration. Further, acceptor dynamics reveals the fact that direct Forster transfer dominates in viscous media.
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Misra et al. (2007) studied this question.
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