Tadpole-like cells of Sporolactobacillus inulinus are nothing but partial spheroplasts formed by the imbalance between cell-elongation and cell-wall formation. This phenomenon was termed auto-spheroplastization. By applying flavin-linked lactate dehydrogenase technique, surface of this organism was found to act as a barrier against the invasion of self-producing D(-)- lactic acid. Auto-spheroplastization can also conveniently be used for showing the actual osmotic barrier located on a cytoplasmic membrane.
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KITAHARA et al. (1972) studied this question.
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