Synthetic phosphoethanolamine (Pho-s) is a monophosphoester ester with anti-inflammatory and pro-apoptotic properties. Meclizine chloridrate (MC) is a histamine H1 receptor blocker that is also able to inhibit cellular respiration. However, MC does not inhibit cellular respiration in isolated mitochondria such as antimycin and rotenone. Methyl--cyclodextrin (MCD) belongs to the -cyclodextrin family, which is capable of removing cholesterol from the plasma membrane. The aim of this study was to evaluate the proliferative effects of meclizine chloridrate and methyl--cyclodextrin compounds associated with synthetic phosphoethanolamine in a triple-negative human breast tumor line, MDA-MB-231 Cell viability of the tumor line and normal cells FN1 was evaluated by MTT colorimetric test; the production of free radicals was determined by lipoperoxidation (LPO) test; and the percentage of cell cycle phases and proliferative index was evaluated by flow cytometry. Cell viability demonstrated a significant decrease with the treatments of MCD, MC and Pho-s associated with MC. The production of free radicals decreases significantly in all treatments. In addition, a significant increase of DNA fragment and decrease in G0/G1 cell cycle phase were observed in cellular percentage with concentrations of 20 and 30 mM of Pho-s in association with MC and MCD, respectively.
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Silva et al. (2019) studied this question.
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