Summary Various procedures for the permanganate oxidation of fats have been tried on purified model fatty acid esters. It was found that all the procedures tested except Kartha's procedure caused considerable loss of ester groups. Kartha's procedure, on the contrary, caused ester groups to be synthesized during the oxidation. It was concluded that none of the oxidation procedures that were tried is a very reliable method for the study of glyceride structure in fats. However the limitations which were discovered in Kartha's procedure could not be shown to invalidate Kartha's hypothesis of glyceride structure.
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Eshelman et al. (1958) studied this question.
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