Blood Orange Juice Authentication Using Cinnamic Acid Derivatives. Variety Differentiations Associated with Flavanone Glycoside Content

A phenyl propanoid glycoside, cinnamoyl-β- d -glucopyranoside (CIN), characteristic of blood oranges, was isolated by column and thin layer chromatographies. The structure of this compound was established using 1 H and 13 C NMR spectral data and by analysis of its components upon hydrolysis. Flavanone glycosides (FGs) [narirutin (NAT), hesperidin (HES), and didymin (DID)] and cinnamic derivatives [CIN and trans -cinnamic acid (TCA)] of four blood orange varieties (47 samples) were analyzed by HPLC. Differences in the polyphenol profiles clearly showed variety differentiation.